Use of plasmid analysis and determination of aminoglycoside-modifying enzymes to characterize isolates from an outbreak of methicillin-resistant Staphylococcus aureus
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چکیده
منابع مشابه
Identification of aminoglycoside-modifying enzymes by susceptibility testing: epidemiology of methicillin-resistant Staphylococcus aureus in Japan.
A multiple-primer PCR was used to identify genes encoding aminoglycoside-modifying enzymes in 381 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). The technique used three sets of primers delineating specific DNA fragments of the aph(3')-III, ant(4')-I, and aac(6')-aph(2") genes, which influence the MICs of gentamicin, tobramycin, and lividomycin. Isolates with none of t...
متن کاملComparison of Biofilm Formation between Methicillin-Resistant and Methicillin-Susceptible Isolates of Staphylococcus aureus
Background: The aim of this study was to compare the biofilm formation and the prevalence of biofilm-associated genes between the isolates of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus. Methods: In total, 209 S. aureus isolates were collected. The antibiotic susceptibility test was conducted using nine antibiotics according to the guidelines of Clinica...
متن کاملDetection of icaABCD Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus from Patients in Iran
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that causes several nosocomial or community-acquired infections. Adhesion to surfaces and subsequent biofilm formation are the major phases of a staphylococcal infection. The aim of this study was to detect the presence of icaABCD genes in clinical isolates of MRSA. Methods: A total of 110 clinical Staphyl...
متن کاملfrequency of aminoglycoside-resistance genes in methicillin-resistant staphylococcus aureus (mrsa) isolates from hospitalized patients
conclusions the present study showed a high prevalence of aminoglycoside-resistance genes among mrsa isolates in two cities in iran. results the results of drug susceptibility testing showed that the highest rate of resistance was against erythromycin in tehran (84.4%) and gentamicin (71.7%) in sari. all isolates were sensitive to vancomycin, and all strains harbored the meca gene. the aac (6’)...
متن کاملDetection of icaABCD Genes and Biofilm Formation in Clinical Isolates of Methicillin Resistant Staphylococcus aureus
Background & Objectives: Methicillin resistance Staphylococcus aureus (MRSA) is one of the most important pathogens that causes several nosocomial and community infections. Adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection. The aims of this study were to determine presence oficaABCD genes and relation to the biofilm formation in of...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1989
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.27.11.2535-2538.1989